Figure 2 | Scientific Reports

Figure 2

From: Prostate cancer cell-platelet bidirectional signaling promotes calcium mobilization, invasion and apoptotic resistance via distinct receptor-ligand pairs

Figure 2

PCa cell lines MDA PCa 2b and RC77 T/E activate platelets. (A) Representative flow cytometry dot plots for surface expression of two platelet activation markers, activated integrin αIIbβ3 (PAC-1) and P-selectin (CD62P). Quantification of flow cytometry of platelets incubated with (B) MDA PCa 2b or (C) RC77 T/E cells for 24 h at a cell:platelet ratio of 1:1000. Data presented as the mean ± SEM of n = 5–6 independent determinations and analyzed by Student’s t-test, *P < 0.05. (D) Representative plot of calcium mobilization in platelets over 90 min following addition of MDA PCa 2b (MDA) cells at the indicated cell:platelet ratios. Red arrow indicates addition of MDA cells. A.U., arbitrary units. (E) Data presented as the mean ± SEM of n = 4–7 independent determinations at the indicated MDA cell:platelet (PLT) ratios and analyzed by ANOVA and Dunnett’s or Tukey’s post-hoc test, *P < 0.05.

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