Table 1 Secretomic analysis of HBD2-stimulated HaCaT cells.

Protein code	Gene	Log2 fold change	p.mod	Protein name
O15263	DEFB4A	10.96	0.00	Beta-defensin 4A
Q9NX55	HYPK	1.37	0.04	Huntingtin-interacting protein K
P01833	PIGR	1.28	0.03	Polymeric immunoglobulin receptor
O95861	BPNT1	1.03	0.04	3'(2'),5'-bisphosphate nucleotidase 1
P59666	DEFA1/3	1.01	0.04	Neutrophil defensin 3
Q96CX2	KCTD12	0.94	0.01	BTB/POZ domain-containing protein KCTD12
O43920	NDUFS5	0.89	0.03	NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
P42330	AKR1C3	− 1.04	0.02	Aldo–keto reductase family 1 member C3
Q92878	RAD50	− 1.07	0.01	DNA repair protein RAD50
Q15046	KARS	− 1.10	0.04	Lysine–tRNA ligase
P02794	FTH1	− 1.12	0.02	Ferritin heavy chain
P81605	DCD	− 1.13	0.02	Dermcidin
P14927	UQCRB	− 1.16	0.04	Cytochrome b-c1 complex subunit 7
Q9NWH9	SLTM	− 1.16	0.00	SAFB-like transcription modulator
P55786	NPEPPS	− 1.24	0.01	Puromycin-sensitive aminopeptidase
P62841	RPS15	− 1.24	0.01	40S ribosomal protein S15
P38919	E1F4A3	− 1.36	0.05	Eukaryotic initiation factor 4A-III
P46937	YAP1	− 1.43	0.01	Transcriptional coactivator YAP1
Q15417	CNN3	− 1.72	0.01	Calponin-3
P35527	KRT9	− 2.34	0.02	Keratin, type I cytoskeletal 9

HaCaT cell monolayers were treated with 0.5 μg/ml HBD2, or vehicle control, in serum free media for 48 h before supernatants were collected, centrifuged at 3000 rpm for 5 min, and stored at -80 °C prior to analysis; n = 5 per condition. Log fold changes and p values were generated using LIMMA pathway⁴³. Table shows proteins with the greatest differences in detection levels between control and HBD2 treated conditions, listed by fold change, largest to smallest, with Uniprot protein code, gene code, fold change, p.mod value, protein name and brief description of function. Protein descriptions made using UniProt database information⁴⁶.

Quick links

Search