Table 4 Proteomic analysis of HBD2-mediated protection from V8 damage in HaCaT cells.

Protein code	Gene	Log2 fold change	p.mod	Protein name
Q16877	PFKFB4	3.12	0.02	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
P40199	CEACAM6	1.31	0.00	Carcinoembryonic antigen-related cell adhesion molecule 6
Q9Y6Y0	IVNS1ABP	1.21	0.00	Influenza virus NS1A-binding protein
Q8IVL5	LEPREL1	1.21	0.03	Prolyl 3-hydroxylase 2
Q9NPA0	EMC7	1.12	0.03	ER membrane protein complex subunit 7
Q6PD74	AAGAB	1.04	0.04	Alpha- and gamma-adaptin-binding protein p34
Q9ULW0	TPX2	1.00	0.04	Targeting protein for Xklp2
Q12873	CHD3	-2.20	0.03	Chromodomain-helicase-DNA-binding protein 3
Q8IYT4	KATNAL2	-2.09	0.01	Katanin p60 ATPase-containing subunit A-like 2
Q15388	TOMM20	-1.57	0.04	Mitochondrial import receptor subunit TOM20 homolog
P23511	NFYA	-1.47	0.03	Nuclear transcription factor Y subunit alpha
Q14108	SCARB2	-1.45	0.03	Lysosome membrane protein 2
Q9Y281	CFL2	-1.38	0.04	Cofilin-2
Q9UPU7	TBC1D2B	-1.33	0.05	TBC1 domain family member 2B
Q2TBE0	CWF19L2	-1.26	0.02	CWF19-like protein 2
O60888	CUTA	-1.14	0.05	Protein CutA
P80188	LCN2	-1.07	0.04	Neutrophil gelatinase-associated lipocalin
Q9UNN8	PROCR	-1.06	0.01	Endothelial protein C receptor
Q12933	TRAF2	-1.01	0.03	TNF receptor-associated factor 2

HaCaT cell monolayers were treated with 0.5 μg/ml HBD2 in serum free media for 24 h, before addition of 5 μg/ml V8, or vehicle control, for a further 24 h. Cells were then collected and centrifuged at 3000 rpm for 5 min. Dry cell pellet stored at -80 °C prior to analysis. n = 5 per condition. Log fold changes and p values generated using LIMMA pathway⁴³. Table shows proteins with the greatest differences in detection levels between HBD2-protected and unprotected cells after exposure to V8, largest to smallest with Uniprot protein code, gene code, fold change, p.mod value, protein name and brief description of function. Protein descriptions made using UniProt database information⁴⁶.

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