Figure 2 | Scientific Reports

Figure 2

From: Patterns of Arc mRNA expression in the rat brain following dual recall of fear- and reward-based socially acquired information

Figure 2

Overview of Experiment Design. This figure outlines the treatment of rats on each day of the experiment from the first day of food restriction on. Following a period of food restriction, demonstrators were fear conditioned to a conditioned stimulus (CS). 24 h later, observers underwent with their demonstrator fear conditioning by-proxy followed shortly by socially transmitted food preference acquisition. Controls received CS exposure the following day with no demonstrator present. Finally, 48 h post-observational learning, all observers and demonstrators underwent a choice test/were given access to the demonstrated food. Controls received plain powdered food for this time period. All rats were then returned to their home cage for 10 min before being returned to the fear conditioning chambers and played a single CS. All rats were subsequently euthanized, and their brains were processed for Arc mRNA expression.

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