Figure 3
Comparison of tissue preservation condition using ex vivo developing submandibular gland culture. (a) Time course representation of tissue preservation at 37, 25, and 4 °C for 7 days, followed by a recovery period at 37 °C for 2 days. SMG: submandibular glands, T0: preservation temperature starting point, T1: preservation temperature endpoint, T2: recovery temperature endpoint. (b) Photographic analysis of cultured E13.5 submandibular glands in low-temperature preservation conditions represented in (a). Scale bars, 200 µm. (c) Changes in the area of cultured E13.5 submandibular gland buds at 37, 25, or 4 °C (n = 7). ns, p > 0.05: *p < 0.05. Error bars represent the mean ± SD.
