Table 4 Sequential extraction of Zn, Cu and Mn from faecal samples collected on day 14.

From: The impact of feeding supplemental minerals to sheep on the return of micronutrients to pasture via urine and faeces

Extraction steps

Elements

IL

IH

OL

OH

P values

Mean ± SE (mg kg−1)

Form

Dose

Form × dose

Step 1: Exchangeable, water- and acid-soluble

Zn

115 ± 12.4

119 ± 20.0

113 ± 12.8

114 ± 7.9

0.767

0.852

0.900

Cu

2.44 ± 0.093

2.67 ± 1.112

2.32 ± 0.172

2.43 ± 0.143

0.163

0.185

0.672

Mn

307 ± 5.2

295 ± 8.0

310 ± 12.1

330 ± 16.0

0.102

0.732

0.165

Step 2: Reducible

Zn

138 ± 7.0

149 ± 23.2

147 ± 12.6

143 ± 4.0

0.902

0.745

0.539

Cu

8.36 ± 0.500

7.91 ± 0.391

7.90 ± 0.456

7.76 ± 0.285

0.378

0.396

0.650

Mn

64.8 ± 3.77

60.1 ± 2.96

62.1 ± 2.83

63.0 ± 2.27

0.988

0.529

0.355

Step 3: Oxidizable

Zn

15.8 ± 3.29

25.4 ± 2.32

20.0 ± 1.42

19.0 ± 1.01

0.012*

0.736

0.898

Cu

22.1 ± 0.74

22.3 ± 0.85

21.1 ± 0.94

22.1 ± 0.89

0.565

0.551

0.701

Mn

12.1 ± 2.16

9.99 ± 1.425

12.6 ± 0.88

10.1 ± 0.82

0.831

0.096

0.865

Step 4: Residual

Zn

5.81 ± 0.716

5.13 ± 0.374

4.57 ± 0.465

4.54 ± 0.261

0.054

0.428

0.463

Cu

6.61 ± 0.143

6.90 ± 0.285

6.01 ± 0.893

6.70 ± 0.362

0.391

0.293

0.660

Mn

2.46 ± 0.160

1.95 ± 0.149

1.98 ± 0.185

1.80 ± 0.130

0.042*

0.027*

0.263

  1. Significant values are in bold.
  2. OH organic minerals offered at higher doses, OL organic minerals offered at lower doses, IH inorganic minerals offered at higher doses, IL inorganic minerals offered at lower doses. The symbol ‘*’ indicate statistical significance of ANOVA test at p-value < 0.05.
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