Figure 3
From: Fungal cell barriers and organelles are disrupted by polyhexamethylene biguanide (PHMB)
Fluorescence imaging showing the reduction of Con A- membrane fluorescence following exposure to increasing concentrations of PHMB. (A) S. cerevisiae cultures were treated with PHMB-rhodamine at 4 µg/ml and 8 µg/ml (B) C. albicans cultures were treated with 8 µg/ml and 12 µg/ml. Cultures were incubated at room temperature for 4 h and counter-stained with Con A-Alexa Fluor 488. Untreated control = growth media only. Images show quenching of membrane fluorescence intensity with increasing PHMB concentration. Graphs show measured fluorescence intensity of sampled cells (n = 20) at four symmetrical points along the cell membrane and averaged (mean ± SD). Membrane fluorescence was analysed by RM One-way ANOVA followed by Tukey's multiple comparison test.
