Figure 6
From: Fungal cell barriers and organelles are disrupted by polyhexamethylene biguanide (PHMB)
Confocal imaging of S. cerevisiae incubated with PHMB. S. cerevisiae were treated with PHMB-rhodamine (4 µg/ml) for 4 h at room temp. Cells were counterstained with DAPI and Con A-Alexa Fluor 488 and imaged by confocal microscopy. Top panels: Confocal images before (left) and after (right) image processing by SRRF. Bottom-left panel: Cross-sectional view of confocal Z-stacks of S. cerevisiae (59 slices). Images show PHMB-rhodamine accumulation within the cytosol and co-localisation with the nucleus (DAPI). The graph confirms high intracellular accumulation of PHMB-rhodamine.
