Figure 4
From: Digestive enzymes and sphingomyelinase D in spiders without venom (Uloboridae)
Spider venom and midgut SMaseD multiple sequence alignment and their classification by cysteine conservation. SMaseD of class I, class IIa and class IIb from L. venom gland were aligned against SMaseD from abdomen A. geniculata, midgut N. cruentata, digestive fluid S. mimosarum, and whole body S. dumicola and O. yesoensis in multiple sequence alignment (MUSCLE software), to classify the SMaseD found in the midgut/digestive fluid of spiders. Sequences with only one disulfide bond between Cys97 and Cys103 are classified as “Class I SMaseD” and sequences with an additional disulfide bond between Cys99 and Cys 250 are classified as “Class II SMaseD''. Red box: 1º disulfide bond cysteine conservation; Purple box: 2º disulfide bond cysteine conservation; Blue line/arrow; columns of amino acids positions hidden (95–108 and 240–253). Midgut/digestive fluid SMaseD sequences only conserve the four cysteine residues (Cys97, Cys99, Cys 103, and Cys 250). Alignment was generated in Jalview (https://www.jalview.org)81.
