Figure 5
From: Digestive enzymes and sphingomyelinase D in spiders without venom (Uloboridae)
Spider venom and midgut SMaseD multiple sequence alignment, their classification by glycine and proline substitutions, and conservation of residues involved in catalysis. SMaseD of class I, class IIa and class IIb from L. venom gland were aligned against SMaseD from abdomen A. geniculata, midgut N. cruentata, digestive fluid S. mimosarum, and whole body S. dumicola and O. yesoensis in multiple sequence alignment (MUSCLE software) 80, to classify the SMaseD found in midgut/digestive fluid of spiders. Most of the class I and class IIa SMaseD conserve the glycine and proline (purple highlighted and red arrowed indicated) residues, and class IIb SMaseD has a substitution in glycine/proline residues. As well, the conservation of histidines (His57 and His93), glutamic acid (Glu77), aspartic acid (Asp79), lysine (Lys139), tyrosine (Tyr278), and tryptophan (Trp280), involved in ion magnesium coordination and substrate recognition. Purple box: conserved histidine, glutamic acid, aspartic acid, lysine, glycine, and proline; Green box: conserved tyrosine; Blue box: conserved tryptophan. Midgut/digestive fluid SMaseD sequences only substitute the glycine residue (Gly141). Alignment was generated in Jalview (https://www.jalview.org81.
