Table 1 Detection of zoonotic Influenza A strains by the QIAstat-Dx Respiratory SARS-CoV-2 Panel.

From: Detecting zoonotic Influenza A using QIAstat-Dx Respiratory SARS-CoV-2 panel for pandemic preparedness

Source

Flu A strain

gBlocks Combination

QIAstat-Dx Generic Influenza A detection

QIAstat-Dx Seasonal H1 subtype detection

QIAstat-Dx H1N1pdm09 subtype detection

Strain number

Strain name

Ct

EPF

Ct

EPF

Ct

EPF

swine “G4” Eurasian (EA) avian-like H1N1 Influenza A virus 6

1

A/swine/Anhui/0202/2018

#1

30.2

109,294

Neg

Neg

Neg

Neg

2

A/swine/Anhui/0203/2018

#1

30.2

109,294

Neg

Neg

Neg

Neg

3

A/swine/Beijing/0301/2018

#2

30.1

143,172

Neg

Neg

Neg

Neg

4

A/swine/Hebei/0113/2017

#3

30.3

67,620

Neg

Neg

Neg

Neg

5

A/swine/Hebei/0116/2017

#4

29.5

67,145

Neg

Neg

Neg

Neg

6

A/swine/Hebei/0221/2017

#5

28.5

45,229

Neg

Neg

Neg

Neg

7

A/swine/Heilongjiang/0110/2017

#6

30.3

71,669

Neg

Neg

Neg

Neg

8

A/swine/Heilongjiang/0140/2017

#7

32.2

88,997

Neg

Neg

Neg

Neg

9

A/swine/Heilongjiang/1214/2016

#6

30.3

71,669

Neg

Neg

Neg

Neg

10

A/swine/Henan/SN10/2018

#8

30.9

96,817

Neg

Neg

Neg

Neg

11

A/swine/Henan/SN11/2018

#8

30.9

96,817

Neg

Neg

Neg

Neg

12

A/swine/Henan/SN12/2018

#8

30.9

96,817

Neg

Neg

Neg

Neg

13

A/swine/Henan/SN13/2018

#8

30.9

96,817

Neg

Neg

Neg

Neg

14

A/swine/Jiangsu/J004/2018

#1

30.2

109,294

Neg

Neg

Neg

Neg

15

A/swine/Jiangsu/J005/2018

#1

30.2

109,294

Neg

Neg

Neg

Neg

16

A/swine/Jiangsu/J006/2018

#1

30.2

109,294

Neg

Neg

Neg

Neg

17

A/swine/Jilin/21/2016

#9

30.5

149,619

Neg

Neg

Neg

Neg

18

A/swine/Jilin/23/2016

#9

30.5

149,619

Neg

Neg

Neg

Neg

19

A/swine/Jilin/29/2016

#9

30.5

149,619

Neg

Neg

Neg

Neg

20

A/swine/Shandong/0334/2017

#10

29.0

92,841

Neg

Neg

Neg

Neg

21

A/swine/Shandong/0336/2017

#6

30.3

71,669

Neg

Neg

Neg

Neg

22

A/swine/Shandong/0337/2017

#6

30.3

71,669

Neg

Neg

Neg

Neg

23

A/swine/Shandong/1203/2016

#11

30.2

75,277

Neg

Neg

Neg

Neg

24

A/swine/Shandong/1207/2016

#6

30.3

71,669

Neg

Neg

Neg

Neg

25

A/swine/Shandong/16/2016

#9

30.5

149,619

Neg

Neg

Neg

Neg

26

A/swine/Shandong/36/2016

#9

30.5

149,619

Neg

Neg

Neg

Neg

27

A/swine/Shandong/9/2016

#9

30.5

149,619

Neg

Neg

Neg

Neg

28

A/swine/Shandong/JM78/2017

#12

30.3

117,056

Neg

Neg

Neg

Neg

29

A/swine/Shandong/LY142/2017

#13

30.8

120,913

Neg

Neg

Neg

Neg

2020 candidate vaccine viruses (CVVs) 5

30

A/Hessen/47/2020|H1N1

#14

31.0

137,759

Neg

Neg

Neg

Neg

31

A/Netherlands/3315/2016|H1N1

#15

28.5

94,076

Neg

Neg

Neg

Neg

32

A/Anhui-Lujiang/39/2018|H9N2

#16

31.6

124,115

Neg

Neg

Neg

Neg

33

A/Hong_Kong/308/2014|H9N2

#17

29.9

148,953

Neg

Neg

Neg

Neg

Controls1

C1

A/H1N1/Brisbane/59/07

n/a

14.9

190,941

15.0

81,471

Neg

Neg

C2

A/Virginia/ATCC1/2009

n/a

15.3

188,178

Neg

Neg

18.0

41,804

  1. A set of recently reported H9, H1 spillover, and the G4 EA Influenza A strains were detected using the QIAstat-Dx Respiratory SARS-CoV-2 Panel, using commercial synthetic gBlock dsDNA sequences. All the recently recorded H9 and H1 zoonotic spillover strains and all the G4 EA Influenza A strains were successfully detected. Negative results for three human seasonal IAV’s (H1, H3 and H1N1 pandemic) indicate a high specificity in the detection assay.
  2. EPF End-point-fluorescence. Neg Negative result.
  3. 1C1 and C2 correspond to Control 1 (Seasonal human H1 subtype; Zeptometrix, Catalogue ID: 0,810,244) and Control 2 (H1N1pdm09 human subtype; ATCC, Catalogue ID: VR-1736).
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