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Figure 1

From: Induction of primordial germ cell-like cells from common marmoset embryonic stem cells by inhibition of WNT and retinoic acid signaling

Figure 1

Induction of BTNG-positive cells by exogenous SOX17 expression with BMP4. (A) Morphology of cjESCs cultured on MEF in DK20FA and PESFA. Scale bar, 200 μm. (B) PCA of the gene expression profiles of pluripotent cell populations in common marmosets and humans. The genes used for the PCA were 839 ortholog genes annotated in both the common marmosets and humans, out of 886 differentially expressed genes between human naïve and primed ESCs30. The transcriptome profiles of common marmoset blastocysts, epiblasts and naïve ESCs were used31. (C) A schematic time course for PGCLC induction with or without exogenous SOX17 expression and BMP4. (D) FACS analysis of BTNG-positive cell induction with overexpression of transcription factors (TFs) and BLSE at day 4. (E) FACS analysis of BTNG-positive cell induction with overexpression of TFs and LSE at day 4.

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