Figure 2

Dissection of molecular pathways downstream of SOX17 and BMP4. (A) Differentiation trajectories during the induction of BTNG-positive cells with SOX17 + BLSE and BLSE alone. Shown are PCA plots of cjESCs and cells at 12, 24, 48, or 96 h of culture under the condition indicated. In this series of analyses, cjESCs were maintained under the DK20FA condition. (B) A scatterplot of the Z scores of the genes for the PC1 and 2 axes. The scatterplot was made using the Z scores of the genes for the PC1 and 2 axes shown in (A). Genes with > 2.5 SDs (695 genes) are shown. (C) Heatmap and GO analyses of genes contributing to each PC. The heatmap shows the expression level of genes categorized in the Z score plot in (B). Representative GO terms for each gene group and their p-values are shown at the right. (D) Expression dynamics of representative genes during the induction of BTNG-positive cells with SOX17 + BLSE and BLSE alone. The graphs show the expression levels of genes involved in PGC specification, pluripotency, epithelial-mesenchymal transition (EMT) and mesoderm differentiation during the induction of BTNG-positive cells. Each plot is based on duplicated RNA-seq analyses. The mean values are connected by the line. (E) Differentiation trajectories during the induction of BTNG-positive cells with SOX17 + BLSE and SOX17 + LSE. (F) A scatterplot of the Z scores of the genes for the PC1 and 2 axes. The scatterplot was made using the Z scores of the genes for the PC1 and 2 axes shown in (E). Genes with > 2.5 SDs (651 genes) are shown. (G) Expression dynamics of representative genes during the induction of BTNG-positive cells with SOX17 + BLSE and SOX17 + LSE. Details are the same as in (D). The analyses in this figure were performed using the common genes between marmosets and humans (15,755 genes).