Figure 6
Protein levels of selected alarmins in the bone marrow supernatants derived from aging mice and the effect of Ang-(1–7): (A) representative western blots for S100A8 and S100A9 in the bone marrow supernatants from Young, Old and Ang-(1–7)-treated Old mice. Memcode was used to estimate the total protein. (B) S100A8 monomers were similar in Young and Old groups, Ang-(1–7) increased the levels in the Old compared to the untreated Old group (*P < 0.05, n = 6). No significant differences were observed in dimer levels. (C) S100A9 monomers were similar in all three groups. On the other hand, dimer levels were similar in the Young and Old but Ang-(1–7) decreased the dimer levels in the Old group (*P < 0.05, n = 6). (D,E) Representative western blots of HMGB1 in three different groups of bone marrow supernatants and Ponceau S was used for the estimation of total protein. HMGB1 levels were similar in Young and Old groups. Ang-(1–7) decreased the protein in the Old group (**P < 0.01, n = 6). (F,G) Representative western blots of RAGE in the bone marrow cells derived from three experimental groups with β-actin as an internal control. While the protein levels of mRAGE were similar in all three groups, xRAGE and total RAGE were higher in the Old bone marrow cells compared to the Young (*P < 0.05, n = 6). Ang-(1–7) has no effect on RAGE levels in Old group. One-way ANOVA was used for testing the statistical differences among experimental groups for comparing protein levels.
