Figure 3

Example dataset and workflow for identification of a positive sample with only one positive pool. (a) Schematic of the sample plate with positive (red) and negative (blue) samples and pools (bold outline). (b) N1 droplet counts for the pools. Pool D, which should have been positive, is indicated in red. (c) Data analysis including the positive pools, expected number of positive samples, deconvolution, and rerun data. (d) Outline of workflow when the actual number of positives (3) was less than expected, resulting in the identification of positive sample D1.