Table 3 Mixture experimental design defining proportions of rice straw compost (RSC), soil around goat and sheep stalls (SGS), and termite intestines (TI) and respective values of FPase, CMCase, avicelase, xylanase, and VS removal efficiency of sugarcane bagasse.

From: Taxonomic and enzymatic basis of the cellulolytic microbial consortium KKU-MC1 and its application in enhancing biomethane production

Run	Component (mL)			FPase (IU/mL)^a		Combination effect*	CMCase (IU/mL)^a		Combination effect*	Avicelase (IU/mL) ^a		Combination effect*	Xylanase (IU/mL) ^a		Combination effect*	VS removal efficiency ^a (%)		Combination effect*
	A:	B:	C:	Exp	Cal		Exp	Cal		Exp	Cal		Exp	Cal		Exp	Cal
	RSC	SGS	TI	Exp	Cal		Exp	Cal		Exp	Cal		Exp	Cal		Exp	Cal
1	0.00	0.00	3.00	2.993 ± 0.22	2.993 ± 0.22	–	0.121 ± 0.17	0.121 ± 0.12	–	0.131 ± 0.04	0.131 ± 0.04	–	0.612 ± 0.06	0.612 ± 0.05	–	55.6 ± 0.02	55.6 ± 0.01	–
2	3.00	0.00	0.00	2.788 ± 0.22	2.788 ± 0.22	–	0.061 ± 0.07	0.061 ± 0.07	–	0.059 ± 0.02	0.059 ± 0.02	–	0.521 ± 0.25	0.521 ± 0.25	–	42.3 ± 2.44	42.3 ± 1.25	–
3	0.00	3.00	0.00	2.531 ± 0.22	2.531 ± 0.22	–	0.181 ± 0.26	0.181 ± 0.25	–	0.182 ± 0.03	0.182 ± 0.02	–	0.014 ± 0.03	0.014 ± 0.02	–	42.1 ± 0.67	42.1 ± 0.52	–
4	0.00	1.50	1.50	2.718 ± 0.22	1.381 ± 0.22	1.97 ± 0.05	0.242 ± 0.07	0.076 ± 0.07	3.21 ± 0.05	0.221 ± 0.01	0.078 ± 0.02	2.82 ± 0.03	0.479 ± 0.55	0.157 ± 0.50	3.06 ± 0.01	51.6 ± 0.79	24.4 ± 0.22	2.11 ± 0.25
5	1.50	1.50	0.00	2.635 ± 0.22	1.330 ± 0.22	1.98 ± 0.01	0.173 ± 0.04	0.061 ± 0.02	2.86 ± 0.14	0.164 ± 0.02	0.060 ± 0.02	2.72 ± 0.22	0.014 ± 0.05	0.134 ± 0.02	0.10 ± 0.12	40.2 ± 1.60	21.1 ± 0.50	1.91 ± 0.02
6	1.50	0.00	1.50	0.323 ± 0.22	1.445 ± 0.22	0.22 ± 0.05	0.043 ± 0.02	0.046 ± 0.12	0.95 ± 0.04	0.041 ± 0.01	0.048 ± 0.02	0.86 ± 0.20	0.049 ± 0.03	0.283 ± 0.02	0.17 ± 0.01	40.2 ± 0.16	24.5 ± 0.22	1.64 ± 0.01
7	2.00	0.50	0.50	2.058 ± 0.22	1.390 ± 0.22	1.48 ± 0.02	0.121 ± 0.06	0.046 ± 0.02	2.66 ± 0.15	0.123 ± 0.01	0.046 ± 0.02	2.69 ± 0.02	0.028 ± 0.02	0.226 ± 0.01	0.12 ± 0.01	41.6 ± 0.16	22.2 ± 1.50	1.87 ± 0.02
8	0.50	0.50	2.00	0.988 ± 0.22	1.441 ± 0.22	0.69 ± 0.02	0.058 ± 0.09	0.061 ± 0.20	0.96 ± 0.05	0.153 ± 0.01	0.064 ± 0.01	2.40 ± 0.14	0.465 ± 0.02	0.249 ± 0.01	1.87 ± 0.22	56.2 ± 0.07	25.6 ± 0.07	2.20 ± 0.15
9	0.50	2.00	0.50	2.248 ± 0.11	1.325 ± 0.12	1.70 ± 0.01	0.184 ± 0.03	0.076 ± 0.02	2.44 ± 0.01	0.176 ± 0.03	0.077 ± 0.02	2.30 ± 0.12	0.021 ± 0.01	0.099 ± 0.02	0.21 ± 0.22	42.1 ± 0.21	22.2 ± 0.20	1.90 ± 0.12
10	1.25	1.25	0.50	2.606 ± 0.22	1.358 ± 0.22	1.92 ± 0.02	0.241 ± 0.04	0.061 ± 0.02	3.98 ± 0.02	0.251 ± 0.04	0.061 ± 0.02	4.11 ± 0.01	0.437 ± 0.03	0.162 ± 0.02	2.69 ± 0.01	50.3 ± 0.22	22.2 ± 0.20	2.26 ± 0.02
11	1.00	1.00	1.00	2.806 ± 0.01	1.385 ± 0.01	2.03 ± 0.04	0.262 ± 0.23	0.061 ± 0.22	4.33 ± 0.15	0.259 ± 0.26	0.062 ± 0.22	4.18 ± 0.01	0.599 ± 0.04	0.191 ± 0.23	3.13 ± 0.15	56.7 ± 0.63	23.3 ± 0.50	2.43 ± 0.25

Cal. calculation, Exp. experimental.
*Co-digestion effect > 1 indicates a synergistic effect.
*Co-digestion effect = 1 indicates no effect.
*Co-digestion effect < 1 indicates an antagonistic effect.
^aThe experimental enzyme activity or VS removal efficiency represents the total amount of enzyme activity or VS removal efficiency generated in each proportion from the cultivation process, and the calculated enzyme activity or VS removal efficiency was calculated from the enzyme activity or VS removal efficiency of the individual substrates in each microorganism source (based on mL) contained in the proportions.

Back to article page

Table 3 Mixture experimental design defining proportions of rice straw compost (RSC), soil around goat and sheep stalls (SGS), and termite intestines (TI) and respective values of FPase, CMCase, avicelase, xylanase, and VS removal efficiency of sugarcane bagasse.

Search

Quick links