Figure 1
NMR 2D 1H,15N-HSQC-spectra of 84 µM 15N-labeled Aβ40 peptides before (blue peaks) and after addition of first 42 µM (red peaks) and then 84 µM (teal peaks) Ni(II) acetate. Spectra were recorded at 5 °C in 20 mM sodium phosphate buffer at pH 7.3 (A), at pH 5.6 (B), and at pH 7.3 together with 50 mM SDS detergent (C). The peak intensity in the bar charts is given as the ratio between the crosspeak intensity with added Ni(II) ions relative to the intensity before addition of Ni(II) ions, i.e. I/I0.
