Figure 3
CD spectra of Ni(II) acetate titrated to Aβ peptides in 20 mM phosphate buffer, pH 7.3, at 25 °C. The titrations were conducted either in the presence of SDS micelles (A–C) or in aqueous buffer only (D–F), for three different peptide variants, i.e. 10 µM Aβ40 (A,D), 10 µM Aβ40(NoHis) (B,E), and 5 µM Aβ(4–40) (C,F). The black spectra show Aβ peptides in buffer only. For samples A–C, 50 mM SDS was then added (red spectra). Next, for all samples, Ni(II) acetate was titrated in steps of 2 µM (orange), 4 µM (yellow), 16 µM (turquoise), 56 µM (green), 156 µM (purple), and finally 256 µM (blue spectra).
