Figure 4
Binding curves for Ni(II)·Aβ complexes, derived from the NMR data in Fig. 1A and the CD data in Fig. 3A,C, respectively. The NMR and CD signal intensities are given as the ratio between the intensity with added Ni(II) ions relative to the intensity before addition of Ni(II) ions, i.e. I/I0. Apparent binding affinities were obtained by fitting Eq. (1) to the curves. (A–C) NMR crosspeak intensity vs Ni(II) concentration for three of the crosspeaks/residues in Fig. 1A, i.e. for 84 µM Aβ40 in 20 mM sodium phosphate buffer, pH 7.3 at 5 °C. (D,E) CD intensity at 208 nm vs Ni(II) concentration for the CD data in Fig. 3A,C, i.e. for 10 µM Aβ40 (D) and 5 µM Aβ(4–40) (E) in 20 mM phosphate buffer, pH 7.3 at 25 °C.
