Figure 3

SARS-CoV-2 infection-induced multi-transcriptional signatures associated with various cellular responses. The NGS data of the brain homogenates of 3 and 4 dpi. Venn diagram indicated the overlapping genes with differential expression. The total number of significantly upregulated and downregulated genes when compared to the corresponding findings in the non-infected mice (A). Biological responses enriched in differentially expressed genes were analyzed by Gene Ontology at 4 dpi when compared to the corresponding findings in the non-infected mice. The false discovery process (q value) determined the rank, and the indicated responses are listed after eliminating the redundant genes (B). Heat maps indicating upregulated genes at 3 and 4 days after Hu-1 or B1.617.2 infection. The cellular signal pathways with enriched inflammatory cytokines, type-I IFN responses, apoptosis, autophagy, and necrosis signaling were identified by Gene Ontology (C). The differentially expressed genes presented in each cellular pathway are the combinations of differential expressed genes. Based on the RNA-seq database, the gene ontology and Heat maps were generated using GraphPad Prism 7.0 (https://www.graphpad.com/).