Figure 2
From: SPLIT-PIN software enabling confocal and super-resolution imaging with a virtually closed pinhole
SPLIT-PIN imaging of DNA in non-adherent cell nuclei. (a, b) SPLIT-PIN imaging of fixed U937-PR9 cell nuclei labeled with the DNA dye To-Pro-3. Shown are confocal images acquired at 0.8 A.U. and 0.5. A.U. and the corresponding SPLIT-PIN images. Sections are shown at a mid plane (a) and at a bottom plane (b) of the nucleus. Scale bars 8 μm (a) and 2 μm (b). (c) Line profiles corresponding to the dashed line in (a). (d) Line profile corresponding to the dashed line in (b). (e) Quantification of Resolution (R), Brightness (B) and Noise (N) parameters for the SPLIT-PIN and 0.2 A.U. images shown in (a) using the QuICS algorithm. (f) Quantification of Resolution (R), Brightness (B) and Noise (N) parameters for the SPLIT-PIN and 0.2 A.U. images shown in (b) using the QuICS algorithm.
