Figure 4
From: SPLIT-PIN software enabling confocal and super-resolution imaging with a virtually closed pinhole
SPLIT-PIN imaging of live human intestinal organoids. (a) Bright field image of a sample of intestinal organoids. Scale bar 50 um. (b) Normalized fluorescence intensity as a function of the depth within the specimen. Data are obtained from two independent measurements. (c) Line profiles corresponding to the dashed line in (e). (d) Quantification of Resolution (R), Brightness (B) and Noise (N) parameters for the SPLIT-PIN and 0.2 A.U. images shown in (e) and (f) using the QuICS algorithm. (e) Series of confocal images of intestinal organoids labeled with CellMask Orange Plasma Membrane acquired with tunable pinhole size (4 A.U. and 1 A.U. respectively) and corresponding SPLIT-PIN image. Scale bar 20 um. (f) Confocal image of the same area of the specimen acquired at 0.2 A.U. and with an integration time equal to the total integration time of the confocal series shown in (e).
