Figure 5
Analysis of the liver transcriptome by second-generation sequencing. (A) Volcano map of differentially expressed genes. (B) Heatmap of differentially expressed genes. (C) Bubble diagram of Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. (D) Bar chart of gene ontology analysis. (E) Quantitative polymerase chain reaction results. Each group at least has 3 samples. The map was plotted using TBtools software15 (version 1.0986; https://github.com/CJ-Chen/TBtools/releases) and a free online platform for data visualization (http://www.bioinformatics.com). Differential gene expression and statistical significance were analyzed using DESeq2 package (http://www.bioconductor.org/). The data in (E) are presented as the mean ± SD. ns, no significant difference; **p < 0.01.
