Table 3 Cycling conditions used in the presented study.
Step | COI (Jerry and Pat) | 16S rDNA (16Sar and 16Sbr) | 28S rDNA (Rd3.2a and Rd5b) |
|---|---|---|---|
Initial denaturation | 95 °C for 3 min | 95 °C for 3 min | 95 °C for 3 min |
Number of cycles | 40 | 40 | 40 |
Denaturation | 95 °C for 30 s | 95 °C for 30 s | 95 °C for 30 s |
Annealing | 50 °C for 30 s | 47 °C for 30 s | 56 °C for 30 s |
Extension | 72 °C for 45 s | 72 °C for 45 s | 72 °C for 45 s |
Final extension | 72 °C for 10 min | 72 °C for 10 min | 72 °C for 10 min |
