Figure 3
From: Crystal structure of the CoV-Y domain of SARS-CoV-2 nonstructural protein 3
Sequence conservation among nsp3 CoV-Y homologs. (a) Sequence conservation of the nsp3 CoV-Y domain among seven human coronaviruses and two close relatives of SARS-CoV-2 (see “Methods” and Supplementary Fig. S5 for sequence information). Conservation is shown as a bar graph, with red bars indicating identity among nine CoV-Y homologs. Secondary-structure assignments of CoV-Y from the crystal structure are shown as cylinders (helices) and arrows (β strands). (b) Sequence identity of the Y1–CoV-Y, CoV-Y and four CoV-Y subdomains of the above eight coronavirus nsp3 sequences to those of SARS-CoV-2 nsp3. The ClustalW alignments were performed using DNASTAR Lasergene Suit 8. The identity numbers are shown in a color scale, with red indicating conservation above 95%. (c) Molecular surface of the CoV-Y domain structure colored according to homologous conservation. Dashed ellipse indicates the two highly conserved regions. (d) Close-up view of the H23 helix of Y2. The six residues with most variation in SARS-CoV-2 isolates are shown in stick representation.
