Figure 7
SB reduces NF-κB RelB levels of protein, mRNA, and binding to motif DNA sequence. (a) Band intensities of NF-κB RelB relative to beta-actin were plotted. In AdCKD kidney, NF-κB RelB protein expression was increased compared to control kidney, which was significantly decreased in AdCKD + SB kidney. (b) NF-κB RelB protein expression was increased in LPS stimulated THP-1 cells compared to control cells, which was significantly decreased in SB treated LPS stimulated THP-1 cells. (c) mRNA levels of NF-κB RelB in AdCKD kidney were increased compared to control, which was significantly decreased in AdCKD + SB. (d) mRNA levels of NF-κB RelB in LPS stimulated THP-1 cells were increased compared to control, which was significantly decreased in SB-treated LPS stimulated cells in a dose dependent manner. (e) mRNA levels of NF-κB RelB in TNF-α stimulated THP-1 cells were increased compared to control, which was significantly decreased in SB-treated TNF-α stimulated cells. (f) NF-κB RelB binding to motif DNA sequence was increased in AdCKD kidney compared to control, which was significantly decreased in AdCKD + SB kidney. (g) NF-κB RelB binding to motif DNA sequence was increased in LPS stimulated THP-1 cells compared to control, which was significantly decreased in SB-treated LPS stimulated cells in a dose dependent manner. (h) NF-κB p65 binding to motif DNA sequence was increased in LPS stimulated THP-1 cells compared to control, which was similar levels compared to SB-treated LPS stimulated cells. **p < 0.01; ***p < 0.001; ns, not significant. Each bar represents mean ± SEM. LPS, lipopolysaccharide; TNF-α, tumor necrosis factor α; SB, sodium benzoate.
