Figure 5

Effects of miR-186-3p/KIF2C axis on NSCLC cell proliferation, migration, and invasion. (a,b); The effects of transfection of miR‐186-3p and si- miR‐186-3p on A549 and A549-DDP cell proliferation were determined by qRT-PCR; (c,d) the effects of miR‐186-3p silencing and overexpression on A549-DDP and A549 cell proliferation were detected by CCK‐8 assays; (e,f) tunel assays were used to detect the cell proliferation and apoptosi; (g,h) A549-DDP cells were co-transfected with pcDNA-KIF2C and miR-186-3p mimics, and A549 cells were co-transfected with si-KIF2C and miR-186-3p inhibitors. After transfection, Western blot were used to detect the changes of β-catenin, p-GSK-3β and p-AKT; (i,l,m) the effects of KIF2C on migration and invasion were evaluated by the wound healing and transwell assays (*P < 0.05, **P < 0.01, and ***P < 0.001).