Table 5 Effects of MSH type, MSH concentration and explant type on responding explants induction (REI), viability of plantlets rate (VPR), shoot proliferation rate (SPR), root proliferation rate (RPR) and callus induction rate (CIR) from the bean plant (P. vulgaris L.).

From: Effects of mammalian sex hormones on in vitro organogenesis of common bean (Phaseolus vulgaris L.)

MSH type

Concentration (mmol L−1)

Explant type

REI (%)*

VPR (%)*

SPR (%)*

RPR (%)*

CIR (%)*

Estrogen

10−10

Embryo

100.0a

100.0a

40.0a

100.0a

100.0a

Hypocotyl

95.0a

77.5b

25.0a

95.0a

37.5b

Plumule

100.0a

100.0a

37.5a

97.5a

100.0a

Radicle

67.5b

2.5c

0.00b

0.00b

0.00c

10−8

Embryo

100.0a

100.0a

50.0a

95.0a

87.5a

Hypocotyl

95.0b

22.5b

12.5bc

77.5b

17.5b

Plumule

100.0a

100.0a

25.0b

97.5a

85.0a

Radicle

80.0c

0.00c

0.00c

0.00c

0.00c

10−6

Embryo

100.0a

100.0a

3.50a

97.5a

70.0b

Hypocotyl

92.5a

20.0b

2.5b

90.0b

15.0c

Plumule

100.0a

100.0a

30.0a

100.0a

85.0a

Radicle

50.0b

0.00c

0.00b

0.00c

0.00d

10−4

Embryo

100.0a

100.0a

3.50a

100.0a

77.5a

Hypocotyl

97.5a

15.0b

0.00b

97.5a

0.00c

Plumule

100.0a

100.0a

7.5b

95.0a

27.5b

Radicle

67.5b

2.5c

0.00b

0.00c

0.00c

Progesterone

10−10

Embryo

100.0a

100.0a

40.0a

100.0a

100.0a

Hypocotyl

95.00a

77.5b

25.0a

95.0a

37.5b

Plumule

100.0a

100.0a

37.5a

97.5a

100.0a

Radicle

67.5b

2.5c

0.00b

0.00b

0.00c

10−8

Embryo

100.0a

100.0a

5.0a

100.0a

82.5a

Hypocotyl

100.0a

17.5b

0.00a

100.0a

20.0b

Plumule

100.0a

100.0a

0.00a

100.0a

17.5b

Radicle

97.50a

2.5c

10.0a

0.00b

0.00c

10−6

Embryo

95.00a

95.0a

30.0a

95.0a

80.0a

Hypocotyl

95.00a

42.5b

7.5bc

92.5a

45.0b

Plumule

100.0a

100.0a

17.5ab

100.0a

87.5a

Radicle

47.5b

2.5c

2.5c

2.5b

15.0c

10−4

Embryo

100.0a

100.0a

30.0a

100.0a

80.0a

Hypocotyl

100.0a

75.0b

15.0a

100.0a

17.5c

Plumule

100.0a

100.0a

32.5a

100.0a

67.5b

Radicle

97.5a

12.5c

12.5a

10.0b

0.00d

Testosterone

10−10

Embryo

100.0a

100.0a

40.0a

100.0a

100.0a

Hypocotyl

95.0a

77.5b

25.0a

95.0a

37.5b

Plumule

100.0a

100.0a

37.5a

97.5a

100.0a

Radicle

67.5b

2.5c

0.00b

0.00b

0.00c

10−8

Embryo

100.0a

10.00a

40.0a

97.5a

97.5a

Hypocotyl

42.5b

97.5a

7.5b

82.5b

30.0b

Plumule

100.0a

100.0a

47.5a

97.5a

87.5a

Radicle

0.00c

57.5b

0.00b

0.00c

0.00c

10−6

Embryo

100.0a

100.0a

55.0a

97.5a

87.5a

Hypocotyl

100.0a

97.5a

20.0b

95.0a

30.0b

Plumule

100.0a

100.0a

55.0a

97.5a

77.5a

Radicle

57.5b

82.5b

7.5b

0.00b

7.5c

10−4

Embryo

7.5c

100.0a

25.0ab

97.5a

95.0a

Hypocotyl

60.0b

95.0a

15.0ab

92.5a

25.0c

Plumule

100.0a

100.0a

37.5a

97.5a

72.5b

Radicle

0.00c

92.5a

10.0b

0.00b

0.00d

17-β-estradiol

10−10

Embryo

100.0a

100.0a

40.0a

100.0a

100.0a

Hypocotyl

95.0a

77.5b

25.0a

95.0a

37.5b

Plumule

100.0a

100.0a

37.5a

97.5a

100.0a

Radicle

67.5b

2.5c

0.00b

0.00b

0.00c

10−8

Embryo

100.0a

100.0a

77.5a

100.0a

72.5a

Hypocotyl

80.0b

62.5b

50.0b

67.5b

65.0a

Plumule

100.0a

100.0a

72.5a

100.0a

62.5a

Radicle

15.0c

0.00c

0.00c

0.00c

0.00c

10−6

Embryo

100.0a

100.0a

70.0a

100.0a

72.5a

Hypocotyl

95.0a

52.5b

20.0b

90.0a

20.0b

Plumule

100.0a

100.0a

5.75a

100.0a

62.5a

Radicle

70.0b

5.0c

27.5b

27.5b

0.00c

10−4

Embryo

100.0a

100.0a

15.0a

100.0a

52.5a

Hypocotyl

100.0a

5.0b

0.00b

92.5a

0.00c

Plumule

100.0a

100.0a

2.5b

97.5a

20.0b

Radicle

57.5b

0.00c

0.00b

0.00b

0.00c

  1. Means within the same column and rows and factors followed by the same letter are not significantly different (p < 0.05). * REI Responding explants induction, VPR viability of plantlets rate, SPR shoot proliferation rate, RPR root proliferation rate and; CIR callus induction.