Figure 1 | Scientific Reports

Figure 1

From: Efficient and accurate KRAS genotyping using digital PCR combined with melting curve analysis for ctDNA from pancreatic cancer patients

Figure 1

Procedure of dPCR combined with melting curve assay. Samples including target DNA were partitioned into microwells, and asymmetric PCR was performed with molecular beacons in the wells. Fluorescence images depending on temperature were captured with three-color filters, and the melting curves were analyzed for each well. Genotyping was performed according to fluorescence intensity, dye color, and Tm.

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