Table 1 Molecular and immunohistochemical findings observed in asymptomatic calves and in cattle with respiratory and enteric manifestations.

Animal#	Age (days)	Clinical manifestation	Types of samples		Results					Type of respiratory infection
			Diarrheic	Respiratory	Molecular detection¹			MCFV IHC
			Diarrheic	Respiratory	BoGHV6	OVGHV2	BCoV	Lung	Intestine
1	60	Asymptomatic	Normal feces ^b	Deep nasal swab	−ve	+ve	−ve	NA	NA	Singular
2	55	Asymptomatic	Normal feces	Deep nasal swab	−ve	+ve	−ve	NA	NA	Singular
3	NP	Respiratory	NS	Deep nasal swab	+ve	+ve	−ve	NA	NA	Dual
4	NP	Respiratory	NS	Deep nasal swab	+ve	+ve	−ve	NA	NA	Dual
5	1	Respiratory	NS	Pulmonary tissue	+ve	−ve ²	−ve	NS	NS	Singular
6^a	2	Respiratory	Normal feces ^b,c	Pulmonary tissue	−ve	+ve ²	−ve	+ve	+ve	Singular
7	7	Respiratory	NS	Pulmonary tissue	−ve	+ve ²	−ve	NS	NS	Singular
8^a	4 years	Respiratory	NS	Pulmonary tissue	−ve	+ve ²,*	−ve	+ve	+ve	Singular
9	40	Respiratory	Normal feces ^b	Deep nasal swab	−ve	+ve	−ve	NA	NA	Singular
10	30	Diarrhea	Liquid feces ^b	Deep nasal swab	−ve	+ve	+ve *	NA	NA	Dual
11	21	Diarrhea	Feces soft	Deep nasal swab	−ve	+ve	−ve	NA	NA	Singular
12	21	Diarrhea	Soft feces	Deep nasal swab	−ve	+ve	−ve	NA	NA	Singular
13	15	Diarrhea	Soft feces	Deep nasal swab	−ve	+ve	−ve	NA	NA	Singular
14	14	Diarrhea	Soft feces	Deep nasal swab	−ve	+ve	−ve	NA	NA	Singular
15	5	Diarrhea	Soft feces ^c	Deep nasal swab	−ve	+ve *	−ve	NA	NA	Singular
16	2–3	Diarrhea	Soft feces	Deep nasal swab	−ve	+ve	−ve	NA	NA	Singular

*Sequenced samples.
^aAnimals that died of pulmonary distress with tissues submitted for routine histopathologic evaluation and fecal samples obtained during gross evaluation.
^bFecal samples infected with BVDV1b.
^cFecal samples infected with Aichivirus. −ve, negative; + ve positive, NP not provided, NS not submitted, NA not applicable.
¹Nucleic acids of BVDV, BoAHV1, BPIV-3, BRSV, P. multocida, H. somni, M. haemolytica, and M. bovis were not amplified from the deep nasal swabs or pulmonary tissues.
²OvGHV2 DNA was amplified from the intestine of these animals with atrophic enteritis.

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