Table 2 Operation Guide (unit: ml).
Blank tube | Standard tube | Assay tube | |
|---|---|---|---|
Precipitant-NaF mixture | 0.5 | – | – |
Lactic acid standard application solution | – | 0.5 | – |
Supernatant | – | – | 0.5 |
4% CuSO4 | 0.1 | 0.1 | 0.1 |
Concentrated sulfuric acid | 3 | 3 | 3 |
Mix well, first heat in a boiling water bath for 5 min, then cool in an ice-water bath for 10 min | |||
1.5% p-Hydroxybiphenyl | 0.1 | 0.1 | 0.1 |
