Figure 4

The correlation between REST and immune cell infiltration in glioma. (A) The infiltration level of various immune cells under different copy numbers of REST in glioma by TIMER2 (http://timer.cistrome.org/). (B) The correlation between REST expression level and infiltration level of immune cells in glioma by TIMER2 (http://timer.cistrome.org/). (C) The correlation between REST expression level and infiltration level of cancer-associated fibroblasts in glioma based on the EPIC, MCPCOUNTER and TIDE algorithms by TIMER2 (http://timer.cistrome.org/). (D) IHC staining (Olympus microscope) and the distribution of REST and CD4 IHC scores (H-SCORE, GraphPad Prism 9.3.1) in normal and glioma tissues by different REST expression. All data are shown as mean ± SD, *P value < 0.05; **P value < 0.01; ***P value < 0.001.