Figure 1
From: Reliable cell retention of mammalian suspension cells in microfluidic cultivation chambers
Structure and cell retention concept of the MSCC device with enhanced cell retention for CHO suspension cell lines. (a) Microfluidic PDMS-glass-based cultivation device. (b) Scanning electron microscopy image of the microfluidic structure illustrating the devices dimensions and trapping barrier. (c) Schematic drawing of the loading procedure and cell retention concept based on a PDMS barrier that is traversable by applying pressure during cell loading but non-traversable by random cellular movement during cultivation. (d) Scanning electron microscopy image of the PDMS barrier located in the cultivation chamber’s entrance.
