Table 1 Overview of recently published microfluidic cultivation devices with enhanced cell retention. The listed approaches are itemized concerning the characteristics of the respective cultivation area, the cell retention method, and the nutrient supply. Additionally, the cultivated cell type is indicated.
From: Reliable cell retention of mammalian suspension cells in microfluidic cultivation chambers
Cultivation area | Retention method | Nutrient supply | Cultivated cell type | References |
|---|---|---|---|---|
Wells | Sedimentation | Diffusive | Mouse hematopoietic cells | Lecault et al.18 |
Wells | Sedimentation | Diffusive | Mouse ovarian cancer cells | Dadgar et al.19 |
Chambers | Pneumatic valves | Convective | Chlamydomonas reinhardtii | Eu et al.20 |
Chambers | Pneumatic valves | Diffusive | Primary murine cells, Murine embryonic stem cells | Dettinger et al.21 |
Chambers | Pneumatic valves | Convective | Human primary mesenchymal stem cells | Gómez-Sjöberg et al.22 |
Chambers | Membrane sealing | Diffusive | Dictyostelium discoideum | Delince et al.23 |
Chambers | Air sealing | None | Salpingoeca rosetta | Halperin et al.24 |
Chambers | Narrow entrances | Diffusive | Chinese hamster ovary cells | Kolnik et al.25 |
