Table 1 Analytical sensitivity of the new qPCR protocol.

From: A new and accurate qPCR protocol to detect plant pathogenic bacteria of the genus ‘Candidatus Liberibacter’ in plants and insects

Spiked samples

Dilutions of spiked samples

Bacterial concentration (DNA copies µl−1)

Cq values of new qPCR without DNA purification

CaLas-infected CC116 sample spiked in C. lemon extract

1/2

89.3

–

1/5

35.7

31.4 ± 0.1

1/10

17.9

32.9 ± 0.2

1/15

11.9

34.2 ± 0.4

1/20

8.9

34.5 ± 0.4

1/40

4.5

37.2 ± 0.2

CaLas-infected CC114 sample spiked in C. reticulata extract

1/2

74.5

–

1/5

29.8

31.2 ± 0.2

1/10

14.9

33.3 ± 0.3

1/15

9.9

33.0 ± 0.0

1/20

7.4

33.2 ± 0.3

1/40

3.7

34.8 ± 0.0

CaLsol-infected PA sample spiked in S. tuberosum extract

1/2

137.8

31.0 ± 0.5

1/5

55.1

32.1 ± 0.3

1/10

27.6

33.0 ± 0.3

1/15

18.4

33.4 ± 0.3

1/20

13.8

34.6 ± 0.2

1/40

6.9

35.1 ± 0.1

  1. Average values \(\left( {{\overline{\text{X}}}_{{{\text{Cq}}}} + {\text{SE}}} \right)\) shown come from triplicate PCR reactions from each template. Spiked samples were prepared by serially diluting CaLas-infected C. lemon and C. reticulata crude extracts or CaLsol-infected S. tuberosum crude extract in respective non-infected crude extracts (v/v). The number of DNA copies per microliter was calculated by the new qPCR method from DNA purification.
Back to article page