Figure 1

Phenotypic changes following Scd1 deletion in Lrig1-expressing epidermal hair follicle cells. (a) RNA in-situ hybridisation of Lrig1 on P19 WT dorsal skin. Polr2A (housekeeping gene) was used as positive control and DapB (Bacterial gene) as negative control. (b) RNA in-situ hybridisation of Scd1, Lrig1 and Duplex Lrig1 and Scd1 on P19 WT Telogen hair follicle. (c) RNA in-situ hybridisation of Scd1, Lrig1 and Duplex in P45 Control Lrig1^+/+; Scd1^fl/fl anagen bulb. (d) RNA in-situ hybridisation of Scd1, Lrig1 and Duplex in P45 Mutant Lrig1-Cre^ERT2/+; Scd1^fl/fl anagen bulb. Purple dotted line denotes boundary of DP cells. Black arrowhead denotes loss of Scd1 transcript expression (scale bar 20 μm). (e) Representative image of shaved Lrig1 Control mouse whole body hair cycle progression (n = 10 mice), post tamoxifen injection at P19. (f) H&E stained sections of Lrig1 Control skin at P21, P40, P48 and P52. (g) Representative image of shaved Lrig1 Mutant mouse whole body hair cycle progression (n = 13 mice), post tamoxifen injection at P19. (h) H&E stained sections of Lrig1 Mutant skin at P21, P40, P48 and P52. Blue arrowhead indicates presence of cellular infiltrates. Black arrowhead indicates exposed club-like hair shaft and missing DP (scale bar 50 μm).