Figure 4
From: Influence of aldo–keto reductase 1C3 polymorphisms in early-onset female psoriasis patients
Cell culture isolated from each AKR1C3 SNPs. AKR1C3 expression in cells isolated from patients with each SNP was quantified by real-time polymerase chain reaction (A) after treatment with BaP (2.5 µM) and (B) after treatment with I3C (200 µM). AKR1C3 expression was significantly decreased in cells with the rs12529 G > C or rs12387 A > G mutations. (C) Expression of keratinocyte differentiation-related genes in real-time PCR of cells derived from each SNP. K5, keratin 5; K10, keratin 10; IVL, involucrin; LOR, loricrin. Cells isolated from patients with rs12529 G > C and rs12387 A > G mutations had increased involucrin and loricrin expression. (D) Immunostaining of AKR1C3 (red) and loricrin (green) in the 3D skin culture constructed with keratinocytes isolated from patients with each AKR1C3 SNP. Abnormal early expression of loricrin from the basal layer was observed in the skin cultures obtained from patients with rs12529 G > C and rs12387 A > G.
