Figure 1
Phenotypic and molecular characterization of RBG1res. (a) Graphical genotypes (chromosomes 1–12) of KO (left), RBG1res-NIL (centre), and NB (right). The white and black rectangles indicate homozygous regions from KO and NB, respectively. The red arrowhead shows the position of RBG1res. The scale bar represents 10 Mb. (b) Differences in resistance to BSR among KO, RBG1res-NIL, and NB. The image shows symptoms of 7-day-old seedlings after inoculation with B. glumae at 108 CFU/ml. The scale bar represents 10 cm. (c) BSR severity of inoculated KO, RBG1res-NIL, and NB. The data are the means ± s.d.s (25 plants × 3 repeats). The different letters indicate significant differences (P < 0.05, Tukey’s honestly significant difference (HSD) test). (d) Candidate region of the RBG1 locus reported previously29 and graphical genotypes (left) and phenotypes (right) of plants containing recombinant DNA between marker loci RM24930 and RM24944. CEN indicates the centromere. (left) Four recombinants were selected from 3252 F2 plants derived from KO × SL535, and these recombinants have segments from chromosome 10 of NB. Black denotes regions homozygous for NB marker alleles; white denotes regions homozygous for KO marker alleles. (right) BSR severity of the recombinant lines KO and SL535. The data are the means ± s.d.s (15 plants × 3 repeats) (Student’s t test). (e) Physical map of the region around RBG1 on chromosome 10 and sequence variations in KO, NB and Mut-W56* in the putative ORF detected in the candidate region at the RBG1 locus (LOC_Os10g04000). aa amino acids, ATG initiation codon, TAG and TGA stop codons.
