Figure 4
RBG1res confers resistance to Burkholderia glumae via negative regulation of ABA. (a) Comparison of the germination response between RBG1res-NIL and KO after treatment with a low concentration of ABA. The shoot and root lengths were measured at 3 days after planting seeds on media supplemented with ABA (1 μM). The data are the shown as the means ± s.d.s; n = 15 (Student’s t test). (b) Image of seeds at 3 days after treatment on media containing a low concentration of ABA (1 μM). The scale bar represents 1 cm. (c) Comparison of OsRab16B expression in embryos of KO and RBG1res-NIL after inoculation with Burkholderia glumae (B. glumae). OsRab16B is an ABA-inducible gene34, and the expression of OsRab16B was normalized to the expression of the ubiquitin gene. The data are the means ± s.d.s; n = 3. (Student’s t test). (d) BSR severity in KO and RBG1res-NIL after treatment with ABA. The seeds were first inoculated with B. glumae, after which ABA or water (control) was sprayed onto 4-day-old seedlings. BSR severity was measured at 3 days after ABA treatment. The data are the means ± s.d.s (25 plants × 3 repeats) (Student’s t test).
