Figure 7
Electron density reconstruction of WT GRB2 dimer in solution calculated from scattering data using DENSS. (A) Orthogonal views with 90° rotation of the proposed full-length SH2/SH2-mediated domain-swapped dimer model superposed onto the electron density reconstruction are shown. One protomer is depicted as in Fig. 1, with the N-terminal SH3 domain in green, central SH2 domain in blue, and C-terminal SH3 domain in orange, linkers in grey, and poly-histidine-tag in red. The other protomer is shown entirely in grey for contrast. (B) The averaged FSC curve as a function of reciprocal electron density resolution is shown. The red, dotted line indicates the resolution limit, where the FSC falls below 0.5.
