Table 1 Summary of ATPase and IVMA results.

From: Virus-free transfection, transient expression, and purification of human cardiac myosin in mammalian muscle cells for biochemical and biophysical assays

Parameters

S1L-eGFP-FLAG

kbasal§ (mean ± SEM, n = 3 S1L preparations, 23 °C)

0.04 ± 0.02 s−1

kcat (mean ± SEM*, n = 3 S1L preparations, 23 °C)

8 ± 1 s−1

KATPase (mean ± SEM*, n = 3 S1L preparations, 23 °C)

80 ± 20 µM

Actin filament velocity (mean ± SEM, 25 °C, n = 30 filaments, preparation 1)1

1513 ± 63 nm/s

Actin filament velocity (mean ± SEM, 25 °C, n = 25 filaments, preparation 2)2

1823 ± 63 nm/s

Fraction of motile filaments (mean ± SEM, 25 °Cn = 5 fields of view, preparation 1)1

60 ± 4

Fraction of motile filaments (mean ± SEM, 25 °C, n = 5 fields of view, preparation 2)2

69 ± 4

  1. §measured (NADH-coupled assay).
  2. *error of the fitting process of Eq. (1) including data from 3 independent preparations (cf. Fig. 3B).
  3. 1Single affinity purification (“deadheading”) applied to preparation 1.
  4. 2Double affinity purification (“deadheading”) applied to preparation 2.
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