Table 1 Summary of the RNA QC metrics according to the sample groups.

From: Technical assessment of different extraction methods and transcriptome profiling of RNA isolated from small volumes of blood

Group

Qubit Concentration (ng/μl; Average ± SD)

RIN (average ± SD)

A260/A230 (average ± SD)

A260/A280 (average ± SD)

Manual untreated 50 μl blood

11.62 ± 2.53

8.16 ± 0.26

1.45 ± 0.55

2.14 ± 0.14

Manual TURBO treated 50 μl blood

7.95 ± 1.39

8.62 ± 0.12

1.99 ± 1.45

1.31 ± 0.09

Automated TURBO treated 16 μl blood

3.50 ± 0.92

7.42 ± 0.51

0.63 ± 0.29

2.19 ± 0.41

Automated TURBO treated 33 μl blood

7.82 ± 0.72

7.29 ± 0.42

1.04 ± 0.38

2.24 ± 0.21

Automated TURBO treated 50 μl blood

8.45 ± 2.27

7.39 ± 0.47

1.2 ± 0.17

2.06 ± 0.23

Automated TURBO treated 66 μl blood

11.61 ± 2.44

7.26 ± 0.47

1.16 ± 0.29

2.12 ± 0.09