Figure 5
From: Sympathetic neurons secrete retrogradely transported TrkA on extracellular vesicles
Inhibition of TrkA-dependent signaling pathways suppresses TrkA+ EV release. (A) Schematic of the downstream pathways activated by TrkA phosphorylation and inhibitors that block them. (B) Schematic of inhibitor and Anti-FLAG-AF488 antibody application to compartmentalized neurons to generate TrkA+ EVs. (C), (D) Quantification of the total number of scatter or fluorescent (Anti-FLAG-AF488+) particles detected after anti-FLAG-AF488 antibody addition to the DA chamber of MFDs containing TrkAFLAG/FLAG SCG neurons treated with the PI3K inhibitor, LY294002. (E), (F) Quantification of the total number of scatter or fluorescent (Anti-FLAG-AF488+) particles detected after anti-FLAG-AF488 antibody addition to the DA chamber of MFDs containing TrkAFLAG/FLAG SCG neurons treated with the PLC-ɣ inhibitor, U73122. Shown is the mean ± SEM for three biological replicates. P values are indicated above the pairwise brackets.
