Figure 5

New allele discovery in relation to the number of individuals sampled. The bars indicate the standard deviation of the estimated mean. The black line includes only individuals with complete MHC IIβ genotypes (successful amplification of both sets of primers: NF2-NR2 and DF2-DR2) (n = 25), while dark and light grey lines include only individuals with successful amplification of NF2-NR2 (MHC IIβ-A and -B, n = 33) and DF2-DR2 (MHC IIβ-C, n = 33), respectively.