Figure 1 | Scientific Reports

Figure 1

From: Mock community as an in situ positive control for amplicon sequencing of microbiotas from the same ecosystem

Figure 1

Experimental design. (a) combinations of mock community and pooled bovine fecal samples considered in the study. Each combination is marked with a different color. Colors reported in this figure match those used in the manuscript Figures. The black circle outline indicates the inclusion of synthetic 16S rDNA molecules (SNA) as PCR spike-ins. (b) schematic representation of the main methodological steps performed in this study. Briefly, bovine fecal samples were pooled into standardized fecal pools. Sample pools were supplemented with the mock community before DNA extraction. Synthetic rDNA molecules were added to DNA samples before PCR amplification. Libraries were sequenced on paired-end runs (2 × 250 bp), using an Illumina MiSeq sequencer. Data analyses included quality processing, generation of SVs and statistical analysis of sequencing data.

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