Table 1 Summary of sample codes and characteristics. For each Pool (BP1, BP2, BP3 n = 3), the sample weight, mock community dose and number of technical replicates are reported.

From: Mock community as an in situ positive control for amplicon sequencing of microbiotas from the same ecosystem

Pool

Replicates

Weight (mg)b

MC dosec

Experimental conditions

Replicate coded

BP1

8

1.25

0

LBM-nMC

BP1-LBM-nMC

8

1.25

0.5

LBM-HMC

BP1-LBM-HMC

8

50.00

0

HBM-nMC

BP1-HBM-nMC

8

50.00

0.5

HBM-HMC

BP1-HBM-HMC

BP2

4

1.25

0.012

LBM-LMC

BP2-LBM-LMC

4

1.25

0.5

LBM-HMC

BP2-LBM-HMC

4

50.00

0

HBM-nMC

BP2-HBM-nMC

4

50.00

0.012

HBM-LMC

BP2-HBM-LMC

4

50.00

0.5

HBM-HMC

BP2-HBM-HMC

BP3

7a

1.25

0

LBM-nMC

BP3-LBM-nMC

7a

1.25

0.012

LBM-LMC

BP3-LBM-LMC

7a

1.25

0.5

LBM-HMC

BP3-LBM-HMC

4

50.00

0

HBM-nMC

BP3-HBM-nMC

4

50.00

0.012

HBM-LMC

BP3-HBM-LMC

4

50.00

0.5

HBM-HMC

BP3-HBM-HMC

  1. a4 technical and 3 biological replicates.
  2. bLow biomass (LBM) correspond to 1.25 mg of pooled bovine fecal sample; High Biomass (HBM) correspond to 50 mg of pooled bovine fecal sample.
  3. cFraction of the recommended dose. One dose (20 μl) includes 2 × 107 cells of each of the two bacteria.
  4. dAmplicon libraries were named by concatenating information for the pool (BP1, BP2, BP3), the sample biomass (LBM: 1.25 mg, HBM: 50.00 mg), the MC dose (nMC: no MC added, LMC: 0.0125 dose, HMC: 0.5 dose).
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