Figure 6

Example of the CIDRE-based tile illumination correction (MIPs, scale bar: 50 \(\upmu \hbox {m}\)): (a) spatial intensity gain model at \(\lambda\) = 618 nm (neurons); (b) gain model at \(\lambda\) = 482 nm (myelinated fibers); (c) original TPFM tile (stack size: 450 \(\upmu {\hbox {m}} \, \times\) 450 \(\upmu {\hbox {m}} \, \times\) 71 \(\upmu {\hbox {m}}\), acquisition time: 3 min 33 s); (d) corrected TPFM tile (zero-light preserved mode³⁰).