Figure 8
From: Fiber enhancement and 3D orientation analysis in label-free two-photon fluorescence microscopy
Frangi-based enhancement of myelinated fibers in the 3D TPFM tile shown in Fig. 6 (MIPs, scale bar: 50 \(\upmu \hbox {m}\)): (a) myelinated fibers autofluorescence (\(\lambda\) = 482 nm), enhanced via the MAGIC preparation protocol27; (b) Frangi’s fiber probability map (\(\alpha =0.001\), \(\beta =1\), scales = 1, 1.25, 1.5 \(\upmu \hbox {m}\)); (c) neuronal bodies (\(\lambda\) = 618 nm); (d) neuron rejection mask (Yen’s thresholding method).
