Figure 2 | Scientific Reports

Figure 2

From: Identifying polymorphic cis-regulatory variants as risk markers for lung carcinogenesis and chemotherapy responses in tobacco smokers from eastern India

Figure 2

Genotyping of (A) rs3764821 of ALDH3B1, (B) rs3748523 of RAD52 and (C) rs5742926 of PMS1 by PCR–RFLP method with a representative chromatogram of Sanger sequencing for each genotype of the rSNPs. For rs3764821, Gel 1: Lane 1,2,4,6 depicts AG genotypes with cut patterns as 240 bp, 198 bp, 42 bp; Lane 3, 7, 8 depicts GG genotypes with 198 bp and 42 bp fragments. For rs3748523, Gel 2: Lane 1,2,3,7 & 8 depicts CC genotypes with 228 bp and 24 bp (not visible) fragments; Lane 4 & 6 depicts GG genotypes as uncut (252 bp) fragments; Lane 8 depicts CG genotype with 252 bp, 228 bp and 24 bp fragments. For rs5742926, Gel 3: Lane 1, 2 & 5 depicts GG genotypes as uncut (340 bp) fragments and Lane 4 depicts GT genotype with 340 bp, 231 bp, 109 bp. A representative chromatogram for heterozygous peak is also provided for each rSNP.

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