Figure 1
Small molecule interactome of S. cerevisiae ATP synthase. (a) Pulldown of ATP synthase complex by HA-6His-tagged Atp6 or the monomers and dimers of ATP synthase extracted by digitonin and separated in two-dimensional BN-SDS-PAGE gel and visualized by silver stain (left panel) or Coomassie (right panel). Gel pieces from region indicated by red frames were cut-off for LC/MS analysis. The experiments were performed many times and representative gels are shown. Original blots/gels are presented in SupplementaryRowImages pages 9 and 10. (b) Venn diagram of the ≤ 20 kDa proteins associated with ATP synthase identified in the interactome analysis from the two different approaches. (c) High confidence ATP synthase interactors identified by MS were connected into a network using the STRING database and visualized using the STRING 106 plugin in Cytoscape. Proteins marked in yellow are known proteins that localize to the mitochondria. Proteins marked in blue are the known ATP synthase subunits identified in the analysis. Proteins marked in green were not previously assigned to be localized to mitochondria. Mco10, Min8 and Yir021w-a are indicated by red frames. (d) Classification of the identified proteins into known complexes and processes.
